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epub Culture of Animal Cells: A Manual of Basic Technique download

by R. Ian Freshney

  • ISBN: 047173991X
  • Author: R. Ian Freshney
  • ePub ver: 1504 kb
  • Fb2 ver: 1504 kb
  • Rating: 4.2 of 5
  • Language: English
  • Publisher: Wiley-Liss; 5 edition (July 26, 2005)
  • Formats: rtf docx azw lrf
  • Category: Math
  • Subcategory: Biological Sciences
epub Culture of Animal Cells: A Manual of Basic Technique download

Basic Cell Culture (Practical Approach Series).

Basic Cell Culture (Practical Approach Series). Introduction to Cell and Tissue Culture: Theory and Technique (Introductory Cell and Molecular Biology Techniques).

In vitro animal cell culture models are powerful tools for studying cellular physiology, initial screening of wide range of drugs, evaluating short and long term effects of different environmental toxins and for basic studies on regenerative medicine. Currently there are very limited in vitro culture model for adult retinal rod and cone photoreceptor neurons; 13-17 the primary sensory neurons of the visual system involved in.

Request permission to reuse content from this site. List of Figures, xix. List of Color Plates, xxiii.

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A SUPERB Manual of Basic Cell CultureTechnique. com User, September 12, 2001. Although many of the recipes used in my field are essentially set, this book allowed for broad learning in the skill of cell culture.

The most complete resource on the techniques, equipment,principles, and practices of animal cell cultureSince publication of the previous edition of this benchmark text, numerous groundbreaking advances have occurred in stem cell research, cloning, tissue engineering, and in vitro toxicity testing. These and other developments have been incorporated into this fully revised and expanded Fifth Edition of Culture of Animal Cells. In addition, to answer the needs of the exponential increase in newcomers to cell culture, particularly in the biopharmaceutical industry, a completely new chapter on training in cell culture technology has been introduced.The most complete resource on the techniques, equipment, principles, and practices of animal cell culture, this text offers a complete background related to growth of animal cells in culture. Beginning with laboratory design, safety, validation and bioethics, then continuing with preparation of media, primary culture and cell lines, through to characterization and authentication, contamination, specialized techniques, and troubleshooting, the coverage includes:* An all-new section of training exercises, separated into basic, intermediate, and advanced procedures, cross-referenced to the relevant protocols* New coverage of stem cells, bioethics, validation, cloning, cell signaling, in vitro toxicity testing, and tissue engineering* An expanded full-color atlas section, with images of primary culture, cell lines, subculture, differentiation, cancer cells and transformation, three-dimensional culture, contamination, and specialized equipment* Enhanced treatment of troubleshooting, with full cross-referencing to the relevant protocols and sections of text* Fully updated references* The clearest, most consistent presentation of step-by-step protocols available* Numerous diagrams, photographs, tables, and charts* Detailed and up-to-date information on reagent preparation and sourcing of materials and equipment, including a fully updated list of suppliers and other resources with Web sitesIndispensable for clinical and biopharmaceutical researchers and scientists, students, trainees, and technicians, this landmark text presents the most accessible and comprehensive introduction available to the culture and experimental manipulation of animal cells.
Comments (7)

Slowly writer
Freshney's Culture of Animal Cells is a great book. It is writen on an easy style, it is concise and complete at the same time and it does teach you all the basics (and a little bit more) of cell culture. When I was doing my masters I didn't have a lot of help with my cell culture experiments and this book was fundamental for my research. The book covers everything, starting from the biology of cultured cells, equipments, design of the lab and asseptic technique and going all the way to primary culture, cloning and selection, cell separation, characterization, differentiation and more. The book (hard cover) is pretty resistant and it easily stands going to the bench (where you surely will be using it a lot). If you are thinking about spending money on a book about cell culture techniques this is the book! Don't think twice.
orginally my teacher required the newer addition but i refused to pay almost 5x more for it so i decided to give this a trry. lucky me the chapters arae the same. in a slightly differernt order but still perefect for my methods in biotechnology class
Outstanding manual for cell culture starters and experts alike.
Description was very accurate. No details were left out. Shipping was quick!
really good book!! it taught me how to use a tissue culture room! however, i work with suspension cells and there is not much about that in the book, just heads up
Received the book right on time as told. Book is in good condition and will never hesitate to recommend a friend. I really love it for the price I paid for.
I purchased this book a year ago to use in a clinical virology laboratory. Although many of the recipes used in my field are essentially set, this book allowed for broad learning in the skill of cell culture. I learned many principles of cell culture that were poorly explained or not mentioned in clinical literature and books.
This book is well laid out, the chapters well written, and the information is comprehensive. Subjects include (besides the usual stuff of cell culture) chapters covering equipment materials, safety, contamination, scale up, a reagent appendix, and supplier's listing.
This book is an excellent choice for the serious beginner. The expert will certainly find this book of value because even experts are only expert at what they do routinely and there is so much to know. This would be a great training source for research or clinical laboratories who perform cell culture.
I've recommended this book to so many people that I thought I'd better come write a review for it. I learned cell culture seven or eight years ago using the 4th edition, which is still excellent. The 5th edition is even better.

Cell culture is both an art and a science, and this book captures both the nitty-gritty details ("How much KCl is in DMEM?") and the aspects that are harder to quantify ("How do I learn to recognize good cell morphology?"). It is both a handy reference guide and an in-depth teaching tool.

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